WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2226482
total bases: 320613408
Q20 bases: 310985103(96.9969%)
Q30 bases: 292729238(91.3029%)

Read1 after filtering:
total reads: 2225390
total bases: 320221917
Q20 bases: 310831408(97.0675%)
Q30 bases: 292640664(91.3868%)

Filtering result:
reads passed filter: 2225390
reads failed due to low quality: 465
reads failed due to too many N: 627
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 83.2489%

JSON report: MontereyBayCA_449-SEQREP.json
HTML report: MontereyBayCA_449-SEQREP.html

fastp -i MontereyBayCA_449-SEQREP.F.fq.gz -o MontereyBayCA_449-SEQREP.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_449-SEQREP.json -h MontereyBayCA_449-SEQREP.html 
fastp v0.20.0, time used: 30 seconds