WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4427300
total bases: 637531200
Q20 bases: 618802118(97.0622%)
Q30 bases: 582146092(91.3126%)

Read1 after filtering:
total reads: 4425013
total bases: 636708269
Q20 bases: 618482145(97.1374%)
Q30 bases: 581965672(91.4022%)

Filtering result:
reads passed filter: 4425013
reads failed due to low quality: 1009
reads failed due to too many N: 1278
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 79.6998%

JSON report: MontereyBayCA_447.json
HTML report: MontereyBayCA_447.html

fastp -i MontereyBayCA_447.F.fq.gz -o MontereyBayCA_447.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_447.json -h MontereyBayCA_447.html 
fastp v0.20.0, time used: 48 seconds