WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2821919
total bases: 406356336
Q20 bases: 395842101(97.4126%)
Q30 bases: 375008252(92.2856%)

Read1 after filtering:
total reads: 2820988
total bases: 405811462
Q20 bases: 395668332(97.5005%)
Q30 bases: 374926794(92.3894%)

Filtering result:
reads passed filter: 2820988
reads failed due to low quality: 317
reads failed due to too many N: 614
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 91.9583%

JSON report: MontereyBayCA_441.json
HTML report: MontereyBayCA_441.html

fastp -i MontereyBayCA_441.F.fq.gz -o MontereyBayCA_441.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_441.json -h MontereyBayCA_441.html 
fastp v0.20.0, time used: 33 seconds