WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1680407
total bases: 241978608
Q20 bases: 235251485(97.22%)
Q30 bases: 222954258(92.138%)

Read1 after filtering:
total reads: 1679874
total bases: 241669335
Q20 bases: 235154364(97.3042%)
Q30 bases: 222908636(92.237%)

Filtering result:
reads passed filter: 1679874
reads failed due to low quality: 210
reads failed due to too many N: 323
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 90.5647%

JSON report: MontereyBayCA_439.json
HTML report: MontereyBayCA_439.html

fastp -i MontereyBayCA_439.F.fq.gz -o MontereyBayCA_439.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_439.json -h MontereyBayCA_439.html 
fastp v0.20.0, time used: 23 seconds