WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2499699
total bases: 359956656
Q20 bases: 349561413(97.1121%)
Q30 bases: 328804840(91.3457%)

Read1 after filtering:
total reads: 2498376
total bases: 359661773
Q20 bases: 349411458(97.15%)
Q30 bases: 328700128(91.3915%)

Filtering result:
reads passed filter: 2498376
reads failed due to low quality: 578
reads failed due to too many N: 745
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.1732%

JSON report: MontereyBayCA_433.json
HTML report: MontereyBayCA_433.html

fastp -i MontereyBayCA_433.F.fq.gz -o MontereyBayCA_433.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_433.json -h MontereyBayCA_433.html 
fastp v0.20.0, time used: 32 seconds