WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2284529
total bases: 328972176
Q20 bases: 320294920(97.3623%)
Q30 bases: 302992181(92.1027%)

Read1 after filtering:
total reads: 2283602
total bases: 328630378
Q20 bases: 320165487(97.4242%)
Q30 bases: 302917497(92.1757%)

Filtering result:
reads passed filter: 2283602
reads failed due to low quality: 422
reads failed due to too many N: 505
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.4391%

JSON report: MontereyBayCA_429.json
HTML report: MontereyBayCA_429.html

fastp -i MontereyBayCA_429.F.fq.gz -o MontereyBayCA_429.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_429.json -h MontereyBayCA_429.html 
fastp v0.20.0, time used: 29 seconds