WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2959594
total bases: 426181536
Q20 bases: 414158897(97.179%)
Q30 bases: 390349679(91.5923%)

Read1 after filtering:
total reads: 2958220
total bases: 425672373
Q20 bases: 413957334(97.2479%)
Q30 bases: 390232799(91.6744%)

Filtering result:
reads passed filter: 2958220
reads failed due to low quality: 506
reads failed due to too many N: 868
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 87.4548%

JSON report: MontereyBayCA_424.json
HTML report: MontereyBayCA_424.html

fastp -i MontereyBayCA_424.F.fq.gz -o MontereyBayCA_424.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_424.json -h MontereyBayCA_424.html 
fastp v0.20.0, time used: 35 seconds