WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4513091
total bases: 649885104
Q20 bases: 631773149(97.2131%)
Q30 bases: 596052139(91.7165%)

Read1 after filtering:
total reads: 4510976
total bases: 649126119
Q20 bases: 631469365(97.2799%)
Q30 bases: 595873399(91.7962%)

Filtering result:
reads passed filter: 4510976
reads failed due to low quality: 791
reads failed due to too many N: 1324
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 84.4088%

JSON report: MontereyBayCA_423.json
HTML report: MontereyBayCA_423.html

fastp -i MontereyBayCA_423.F.fq.gz -o MontereyBayCA_423.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_423.json -h MontereyBayCA_423.html 
fastp v0.20.0, time used: 49 seconds