WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2951665
total bases: 425039760
Q20 bases: 412978830(97.1624%)
Q30 bases: 389603307(91.6628%)

Read1 after filtering:
total reads: 2950414
total bases: 424704710
Q20 bases: 412819336(97.2015%)
Q30 bases: 389494111(91.7094%)

Filtering result:
reads passed filter: 2950414
reads failed due to low quality: 428
reads failed due to too many N: 823
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 88.9341%

JSON report: MontereyBayCA_421.json
HTML report: MontereyBayCA_421.html

fastp -i MontereyBayCA_421.F.fq.gz -o MontereyBayCA_421.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_421.json -h MontereyBayCA_421.html 
fastp v0.20.0, time used: 35 seconds