WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2215406
total bases: 319018464
Q20 bases: 310500567(97.33%)
Q30 bases: 293896168(92.1251%)

Read1 after filtering:
total reads: 2214640
total bases: 318564160
Q20 bases: 310357799(97.424%)
Q30 bases: 293831192(92.2361%)

Filtering result:
reads passed filter: 2214640
reads failed due to low quality: 284
reads failed due to too many N: 482
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 90.647%

JSON report: MontereyBayCA_414.json
HTML report: MontereyBayCA_414.html

fastp -i MontereyBayCA_414.F.fq.gz -o MontereyBayCA_414.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_414.json -h MontereyBayCA_414.html 
fastp v0.20.0, time used: 29 seconds