WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1806169
total bases: 260088336
Q20 bases: 253365503(97.4152%)
Q30 bases: 240226661(92.3635%)

Read1 after filtering:
total reads: 1805564
total bases: 259821545
Q20 bases: 253271593(97.4791%)
Q30 bases: 240176169(92.4389%)

Filtering result:
reads passed filter: 1805564
reads failed due to low quality: 247
reads failed due to too many N: 358
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 90.8401%

JSON report: MontereyBayCA_413.json
HTML report: MontereyBayCA_413.html

fastp -i MontereyBayCA_413.F.fq.gz -o MontereyBayCA_413.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_413.json -h MontereyBayCA_413.html 
fastp v0.20.0, time used: 25 seconds