WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1989922
total bases: 286548768
Q20 bases: 278763774(97.2832%)
Q30 bases: 263857406(92.0812%)

Read1 after filtering:
total reads: 1989210
total bases: 286131988
Q20 bases: 278635515(97.3801%)
Q30 bases: 263801218(92.1956%)

Filtering result:
reads passed filter: 1989210
reads failed due to low quality: 325
reads failed due to too many N: 387
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 89.7432%

JSON report: MontereyBayCA_410.json
HTML report: MontereyBayCA_410.html

fastp -i MontereyBayCA_410.F.fq.gz -o MontereyBayCA_410.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_410.json -h MontereyBayCA_410.html 
fastp v0.20.0, time used: 28 seconds