WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3106907
total bases: 447394608
Q20 bases: 435782912(97.4046%)
Q30 bases: 412756243(92.2578%)

Read1 after filtering:
total reads: 3105844
total bases: 447030236
Q20 bases: 435635213(97.451%)
Q30 bases: 412663942(92.3123%)

Filtering result:
reads passed filter: 3105844
reads failed due to low quality: 385
reads failed due to too many N: 678
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 87.1178%

JSON report: MontereyBayCA_407.json
HTML report: MontereyBayCA_407.html

fastp -i MontereyBayCA_407.F.fq.gz -o MontereyBayCA_407.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_407.json -h MontereyBayCA_407.html 
fastp v0.20.0, time used: 36 seconds