WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2519509
total bases: 362809296
Q20 bases: 352744912(97.226%)
Q30 bases: 333841566(92.0157%)

Read1 after filtering:
total reads: 2518573
total bases: 362278846
Q20 bases: 352579711(97.3227%)
Q30 bases: 333768661(92.1303%)

Filtering result:
reads passed filter: 2518573
reads failed due to low quality: 432
reads failed due to too many N: 504
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 91.6185%

JSON report: MontereyBayCA_403.json
HTML report: MontereyBayCA_403.html

fastp -i MontereyBayCA_403.F.fq.gz -o MontereyBayCA_403.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_403.json -h MontereyBayCA_403.html 
fastp v0.20.0, time used: 33 seconds