WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3670938
total bases: 528615072
Q20 bases: 514489781(97.3279%)
Q30 bases: 486990244(92.1257%)

Read1 after filtering:
total reads: 3669666
total bases: 527907174
Q20 bases: 514261238(97.4151%)
Q30 bases: 486881882(92.2287%)

Filtering result:
reads passed filter: 3669666
reads failed due to low quality: 480
reads failed due to too many N: 792
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 88.8416%

JSON report: MontereyBayCA_400.json
HTML report: MontereyBayCA_400.html

fastp -i MontereyBayCA_400.F.fq.gz -o MontereyBayCA_400.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j MontereyBayCA_400.json -h MontereyBayCA_400.html 
fastp v0.20.0, time used: 41 seconds