WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 2666813 total bases: 384021072 Q20 bases: 363402647(94.6309%) Q30 bases: 332146025(86.4916%) Read1 after filtering: total reads: 2665582 total bases: 382973456 Q20 bases: 363142306(94.8218%) Q30 bases: 332084929(86.7123%) Filtering result: reads passed filter: 2665582 reads failed due to low quality: 895 reads failed due to too many N: 336 reads failed due to too short: 0 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 59.1835% JSON report: DabobBayWA_FG228.json HTML report: DabobBayWA_FG228.html fastp -i DabobBayWA_FG228.F.fq.gz -o DabobBayWA_FG228.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j DabobBayWA_FG228.json -h DabobBayWA_FG228.html fastp v0.20.0, time used: 34 seconds