WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3119018
total bases: 449138592
Q20 bases: 436780866(97.2486%)
Q30 bases: 413031280(91.9608%)

Read1 after filtering:
total reads: 3117769
total bases: 448687643
Q20 bases: 436611009(97.3085%)
Q30 bases: 412932995(92.0313%)

Filtering result:
reads passed filter: 3117769
reads failed due to low quality: 567
reads failed due to too many N: 682
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 87.9725%

JSON report: CypressIslandWA_FG150-SEQREP.json
HTML report: CypressIslandWA_FG150-SEQREP.html

fastp -i CypressIslandWA_FG150-SEQREP.F.fq.gz -o CypressIslandWA_FG150-SEQREP.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG150-SEQREP.json -h CypressIslandWA_FG150-SEQREP.html 
fastp v0.20.0, time used: 36 seconds