WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3375454
total bases: 486065376
Q20 bases: 472721428(97.2547%)
Q30 bases: 447086440(91.9807%)

Read1 after filtering:
total reads: 3374246
total bases: 485604195
Q20 bases: 472550418(97.3118%)
Q30 bases: 446989315(92.0481%)

Filtering result:
reads passed filter: 3374246
reads failed due to low quality: 532
reads failed due to too many N: 676
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 87.095%

JSON report: CypressIslandWA_FG149.json
HTML report: CypressIslandWA_FG149.html

fastp -i CypressIslandWA_FG149.F.fq.gz -o CypressIslandWA_FG149.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG149.json -h CypressIslandWA_FG149.html 
fastp v0.20.0, time used: 39 seconds