WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1947472
total bases: 280435968
Q20 bases: 272586394(97.2009%)
Q30 bases: 257904231(91.9655%)

Read1 after filtering:
total reads: 1946745
total bases: 279951476
Q20 bases: 272442155(97.3176%)
Q30 bases: 257843898(92.1031%)

Filtering result:
reads passed filter: 1946745
reads failed due to low quality: 292
reads failed due to too many N: 435
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 89.4864%

JSON report: CypressIslandWA_FG149-SEQREP.json
HTML report: CypressIslandWA_FG149-SEQREP.html

fastp -i CypressIslandWA_FG149-SEQREP.F.fq.gz -o CypressIslandWA_FG149-SEQREP.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG149-SEQREP.json -h CypressIslandWA_FG149-SEQREP.html 
fastp v0.20.0, time used: 27 seconds