WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3580316
total bases: 515565504
Q20 bases: 487740746(94.6031%)
Q30 bases: 445840316(86.476%)

Read1 after filtering:
total reads: 3578592
total bases: 514595166
Q20 bases: 487474822(94.7298%)
Q30 bases: 445757890(86.623%)

Filtering result:
reads passed filter: 3578592
reads failed due to low quality: 1271
reads failed due to too many N: 453
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 54.2193%

JSON report: CypressIslandWA_FG148.json
HTML report: CypressIslandWA_FG148.html

fastp -i CypressIslandWA_FG148.F.fq.gz -o CypressIslandWA_FG148.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG148.json -h CypressIslandWA_FG148.html 
fastp v0.20.0, time used: 43 seconds