WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2360507
total bases: 339913008
Q20 bases: 321609019(94.6151%)
Q30 bases: 294209484(86.5543%)

Read1 after filtering:
total reads: 2359476
total bases: 338781534
Q20 bases: 321346816(94.8537%)
Q30 bases: 294158577(86.8284%)

Filtering result:
reads passed filter: 2359476
reads failed due to low quality: 750
reads failed due to too many N: 281
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 57.4401%

JSON report: CypressIslandWA_FG147.json
HTML report: CypressIslandWA_FG147.html

fastp -i CypressIslandWA_FG147.F.fq.gz -o CypressIslandWA_FG147.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG147.json -h CypressIslandWA_FG147.html 
fastp v0.20.0, time used: 33 seconds