WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2889430
total bases: 416077920
Q20 bases: 393944817(94.6805%)
Q30 bases: 360297282(86.5937%)

Read1 after filtering:
total reads: 2888390
total bases: 415342182
Q20 bases: 393754539(94.8024%)
Q30 bases: 360244301(86.7343%)

Filtering result:
reads passed filter: 2888390
reads failed due to low quality: 715
reads failed due to too many N: 325
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 56.7106%

JSON report: CypressIslandWA_FG146.json
HTML report: CypressIslandWA_FG146.html

fastp -i CypressIslandWA_FG146.F.fq.gz -o CypressIslandWA_FG146.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG146.json -h CypressIslandWA_FG146.html 
fastp v0.20.0, time used: 38 seconds