WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2826247
total bases: 406979568
Q20 bases: 384610882(94.5037%)
Q30 bases: 351262133(86.3095%)

Read1 after filtering:
total reads: 2825040
total bases: 405726965
Q20 bases: 384314671(94.7225%)
Q30 bases: 351201333(86.561%)

Filtering result:
reads passed filter: 2825040
reads failed due to low quality: 865
reads failed due to too many N: 342
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 56.3034%

JSON report: CypressIslandWA_FG144.json
HTML report: CypressIslandWA_FG144.html

fastp -i CypressIslandWA_FG144.F.fq.gz -o CypressIslandWA_FG144.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG144.json -h CypressIslandWA_FG144.html 
fastp v0.20.0, time used: 37 seconds