WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2634843
total bases: 379417392
Q20 bases: 358782934(94.5615%)
Q30 bases: 327785172(86.3917%)

Read1 after filtering:
total reads: 2633704
total bases: 378163201
Q20 bases: 358488571(94.7973%)
Q30 bases: 327729208(86.6634%)

Filtering result:
reads passed filter: 2633704
reads failed due to low quality: 819
reads failed due to too many N: 320
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 56.8436%

JSON report: CypressIslandWA_FG143.json
HTML report: CypressIslandWA_FG143.html

fastp -i CypressIslandWA_FG143.F.fq.gz -o CypressIslandWA_FG143.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG143.json -h CypressIslandWA_FG143.html 
fastp v0.20.0, time used: 35 seconds