WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2108796
total bases: 303666624
Q20 bases: 287335022(94.6219%)
Q30 bases: 262832076(86.5528%)

Read1 after filtering:
total reads: 2107884
total bases: 302866328
Q20 bases: 287141355(94.8079%)
Q30 bases: 262787427(86.7668%)

Filtering result:
reads passed filter: 2107884
reads failed due to low quality: 666
reads failed due to too many N: 246
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 60.1954%

JSON report: CypressIslandWA_FG142.json
HTML report: CypressIslandWA_FG142.html

fastp -i CypressIslandWA_FG142.F.fq.gz -o CypressIslandWA_FG142.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG142.json -h CypressIslandWA_FG142.html 
fastp v0.20.0, time used: 31 seconds