WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2067485
total bases: 297717840
Q20 bases: 281839723(94.6667%)
Q30 bases: 258020177(86.666%)

Read1 after filtering:
total reads: 2066668
total bases: 297073222
Q20 bases: 281679956(94.8184%)
Q30 bases: 257978257(86.84%)

Filtering result:
reads passed filter: 2066668
reads failed due to low quality: 569
reads failed due to too many N: 248
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 61.078%

JSON report: CypressIslandWA_FG141.json
HTML report: CypressIslandWA_FG141.html

fastp -i CypressIslandWA_FG141.F.fq.gz -o CypressIslandWA_FG141.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG141.json -h CypressIslandWA_FG141.html 
fastp v0.20.0, time used: 29 seconds