WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1870636
total bases: 269371584
Q20 bases: 254572099(94.5059%)
Q30 bases: 232429085(86.2857%)

Read1 after filtering:
total reads: 1869856
total bases: 268869439
Q20 bases: 254438245(94.6326%)
Q30 bases: 232388765(86.4318%)

Filtering result:
reads passed filter: 1869856
reads failed due to low quality: 536
reads failed due to too many N: 244
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 65.1256%

JSON report: CypressIslandWA_FG140.json
HTML report: CypressIslandWA_FG140.html

fastp -i CypressIslandWA_FG140.F.fq.gz -o CypressIslandWA_FG140.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG140.json -h CypressIslandWA_FG140.html 
fastp v0.20.0, time used: 28 seconds