WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2051245
total bases: 295379280
Q20 bases: 279274711(94.5478%)
Q30 bases: 255123890(86.3716%)

Read1 after filtering:
total reads: 2050375
total bases: 294751254
Q20 bases: 279114336(94.6949%)
Q30 bases: 255078717(86.5403%)

Filtering result:
reads passed filter: 2050375
reads failed due to low quality: 594
reads failed due to too many N: 276
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 62.0297%

JSON report: CypressIslandWA_FG136.json
HTML report: CypressIslandWA_FG136.html

fastp -i CypressIslandWA_FG136.F.fq.gz -o CypressIslandWA_FG136.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG136.json -h CypressIslandWA_FG136.html 
fastp v0.20.0, time used: 30 seconds