WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1436582
total bases: 206867808
Q20 bases: 195741500(94.6215%)
Q30 bases: 179076075(86.5655%)

Read1 after filtering:
total reads: 1435990
total bases: 206153799
Q20 bases: 195576205(94.8691%)
Q30 bases: 179045840(86.8506%)

Filtering result:
reads passed filter: 1435990
reads failed due to low quality: 416
reads failed due to too many N: 176
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 64.9845%

JSON report: CypressIslandWA_FG135.json
HTML report: CypressIslandWA_FG135.html

fastp -i CypressIslandWA_FG135.F.fq.gz -o CypressIslandWA_FG135.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG135.json -h CypressIslandWA_FG135.html 
fastp v0.20.0, time used: 24 seconds