WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 4128616
total bases: 594520704
Q20 bases: 578392617(97.2872%)
Q30 bases: 547140823(92.0306%)

Read1 after filtering:
total reads: 4127101
total bases: 593964225
Q20 bases: 578180021(97.3426%)
Q30 bases: 547015235(92.0957%)

Filtering result:
reads passed filter: 4127101
reads failed due to low quality: 617
reads failed due to too many N: 898
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 84.4131%

JSON report: CypressIslandWA_FG134.json
HTML report: CypressIslandWA_FG134.html

fastp -i CypressIslandWA_FG134.F.fq.gz -o CypressIslandWA_FG134.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG134.json -h CypressIslandWA_FG134.html 
fastp v0.20.0, time used: 43 seconds