WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2336065
total bases: 336393360
Q20 bases: 326875200(97.1705%)
Q30 bases: 309051991(91.8722%)

Read1 after filtering:
total reads: 2335192
total bases: 335810556
Q20 bases: 326703199(97.2879%)
Q30 bases: 308981784(92.0107%)

Filtering result:
reads passed filter: 2335192
reads failed due to low quality: 378
reads failed due to too many N: 495
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 85.7335%

JSON report: CypressIslandWA_FG133.json
HTML report: CypressIslandWA_FG133.html

fastp -i CypressIslandWA_FG133.F.fq.gz -o CypressIslandWA_FG133.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG133.json -h CypressIslandWA_FG133.html 
fastp v0.20.0, time used: 31 seconds