WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2294944
total bases: 330471936
Q20 bases: 321000954(97.1341%)
Q30 bases: 303568227(91.859%)

Read1 after filtering:
total reads: 2294147
total bases: 330077867
Q20 bases: 320869956(97.2104%)
Q30 bases: 303503338(91.949%)

Filtering result:
reads passed filter: 2294147
reads failed due to low quality: 335
reads failed due to too many N: 462
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.6861%

JSON report: CypressIslandWA_FG133-SEQREP.json
HTML report: CypressIslandWA_FG133-SEQREP.html

fastp -i CypressIslandWA_FG133-SEQREP.F.fq.gz -o CypressIslandWA_FG133-SEQREP.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG133-SEQREP.json -h CypressIslandWA_FG133-SEQREP.html 
fastp v0.20.0, time used: 30 seconds