WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 1918345
total bases: 276241680
Q20 bases: 261900521(94.8085%)
Q30 bases: 240006131(86.8827%)

Read1 after filtering:
total reads: 1917521
total bases: 275577924
Q20 bases: 261732905(94.976%)
Q30 bases: 239962411(87.0761%)

Filtering result:
reads passed filter: 1917521
reads failed due to low quality: 549
reads failed due to too many N: 275
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 63.6581%

JSON report: CypressIslandWA_FG131.json
HTML report: CypressIslandWA_FG131.html

fastp -i CypressIslandWA_FG131.F.fq.gz -o CypressIslandWA_FG131.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG131.json -h CypressIslandWA_FG131.html 
fastp v0.20.0, time used: 27 seconds