WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2742326
total bases: 394894944
Q20 bases: 374374594(94.8036%)
Q30 bases: 342850724(86.8207%)

Read1 after filtering:
total reads: 2741259
total bases: 393662022
Q20 bases: 374083477(95.0266%)
Q30 bases: 342793892(87.0782%)

Filtering result:
reads passed filter: 2741259
reads failed due to low quality: 711
reads failed due to too many N: 356
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 55.5189%

JSON report: CypressIslandWA_FG129.json
HTML report: CypressIslandWA_FG129.html

fastp -i CypressIslandWA_FG129.F.fq.gz -o CypressIslandWA_FG129.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG129.json -h CypressIslandWA_FG129.html 
fastp v0.20.0, time used: 36 seconds