WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2289828
total bases: 329735232
Q20 bases: 312199386(94.6818%)
Q30 bases: 285750079(86.6605%)

Read1 after filtering:
total reads: 2288839
total bases: 329105313
Q20 bases: 312033302(94.8126%)
Q30 bases: 285700945(86.8114%)

Filtering result:
reads passed filter: 2288839
reads failed due to low quality: 707
reads failed due to too many N: 282
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 57.4755%

JSON report: CypressIslandWA_FG127.json
HTML report: CypressIslandWA_FG127.html

fastp -i CypressIslandWA_FG127.F.fq.gz -o CypressIslandWA_FG127.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG127.json -h CypressIslandWA_FG127.html 
fastp v0.20.0, time used: 33 seconds