WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2800428
total bases: 403261632
Q20 bases: 381810492(94.6806%)
Q30 bases: 349390103(86.641%)

Read1 after filtering:
total reads: 2799228
total bases: 402486916
Q20 bases: 381607167(94.8123%)
Q30 bases: 349331065(86.7931%)

Filtering result:
reads passed filter: 2799228
reads failed due to low quality: 866
reads failed due to too many N: 334
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 57.5189%

JSON report: CypressIslandWA_FG126.json
HTML report: CypressIslandWA_FG126.html

fastp -i CypressIslandWA_FG126.F.fq.gz -o CypressIslandWA_FG126.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG126.json -h CypressIslandWA_FG126.html 
fastp v0.20.0, time used: 37 seconds