WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2590622
total bases: 373049568
Q20 bases: 353489947(94.7568%)
Q30 bases: 323871632(86.8173%)

Read1 after filtering:
total reads: 2589504
total bases: 371838443
Q20 bases: 353206748(94.9893%)
Q30 bases: 323818177(87.0857%)

Filtering result:
reads passed filter: 2589504
reads failed due to low quality: 829
reads failed due to too many N: 289
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 60.2344%

JSON report: CypressIslandWA_FG124.json
HTML report: CypressIslandWA_FG124.html

fastp -i CypressIslandWA_FG124.F.fq.gz -o CypressIslandWA_FG124.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG124.json -h CypressIslandWA_FG124.html 
fastp v0.20.0, time used: 34 seconds