WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3247670
total bases: 467664480
Q20 bases: 442465229(94.6117%)
Q30 bases: 404437146(86.4802%)

Read1 after filtering:
total reads: 3246214
total bases: 466581032
Q20 bases: 442187927(94.7719%)
Q30 bases: 404363864(86.6653%)

Filtering result:
reads passed filter: 3246214
reads failed due to low quality: 1023
reads failed due to too many N: 433
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 52.3968%

JSON report: CypressIslandWA_FG123.json
HTML report: CypressIslandWA_FG123.html

fastp -i CypressIslandWA_FG123.F.fq.gz -o CypressIslandWA_FG123.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG123.json -h CypressIslandWA_FG123.html 
fastp v0.20.0, time used: 40 seconds