WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2455684
total bases: 353618496
Q20 bases: 334767020(94.669%)
Q30 bases: 306373035(86.6394%)

Read1 after filtering:
total reads: 2454692
total bases: 352492132
Q20 bases: 334503098(94.8966%)
Q30 bases: 306321031(86.9015%)

Filtering result:
reads passed filter: 2454692
reads failed due to low quality: 667
reads failed due to too many N: 325
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 54.2445%

JSON report: CypressIslandWA_FG121.json
HTML report: CypressIslandWA_FG121.html

fastp -i CypressIslandWA_FG121.F.fq.gz -o CypressIslandWA_FG121.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG121.json -h CypressIslandWA_FG121.html 
fastp v0.20.0, time used: 32 seconds