WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2444343
total bases: 351985392
Q20 bases: 332933003(94.5872%)
Q30 bases: 304386366(86.477%)

Read1 after filtering:
total reads: 2443295
total bases: 350951670
Q20 bases: 332686410(94.7955%)
Q30 bases: 304333958(86.7168%)

Filtering result:
reads passed filter: 2443295
reads failed due to low quality: 732
reads failed due to too many N: 316
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 56.7579%

JSON report: CypressIslandWA_FG116.json
HTML report: CypressIslandWA_FG116.html

fastp -i CypressIslandWA_FG116.F.fq.gz -o CypressIslandWA_FG116.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG116.json -h CypressIslandWA_FG116.html 
fastp v0.20.0, time used: 38 seconds