WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 1484479 total bases: 213764976 Q20 bases: 202289314(94.6316%) Q30 bases: 185027503(86.5565%) Read1 after filtering: total reads: 1483767 total bases: 213205130 Q20 bases: 202150452(94.815%) Q30 bases: 184993236(86.7677%) Filtering result: reads passed filter: 1483767 reads failed due to low quality: 525 reads failed due to too many N: 187 reads failed due to too short: 0 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 65.1534% JSON report: CypressIslandWA_FG113.json HTML report: CypressIslandWA_FG113.html fastp -i CypressIslandWA_FG113.F.fq.gz -o CypressIslandWA_FG113.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG113.json -h CypressIslandWA_FG113.html fastp v0.20.0, time used: 24 seconds