WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3180510
total bases: 457993440
Q20 bases: 445357374(97.241%)
Q30 bases: 421177209(91.9614%)

Read1 after filtering:
total reads: 3179280
total bases: 457462924
Q20 bases: 445171598(97.3132%)
Q30 bases: 421080498(92.0469%)

Filtering result:
reads passed filter: 3179280
reads failed due to low quality: 554
reads failed due to too many N: 676
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 85.1946%

JSON report: CypressIslandWA_FG110.json
HTML report: CypressIslandWA_FG110.html

fastp -i CypressIslandWA_FG110.F.fq.gz -o CypressIslandWA_FG110.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG110.json -h CypressIslandWA_FG110.html 
fastp v0.20.0, time used: 37 seconds