WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3786878
total bases: 545310432
Q20 bases: 535146724(98.1362%)
Q30 bases: 519047566(95.1839%)

Read1 after filtering:
total reads: 3782969
total bases: 544745385
Q20 bases: 534744951(98.1642%)
Q30 bases: 518707431(95.2202%)

Filtering result:
reads passed filter: 3782969
reads failed due to low quality: 830
reads failed due to too many N: 3079
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 85.351%

JSON report: CypressIslandWA_FG109.json
HTML report: CypressIslandWA_FG109.html

fastp -i CypressIslandWA_FG109.F.fq.gz -o CypressIslandWA_FG109.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG109.json -h CypressIslandWA_FG109.html 
fastp v0.20.0, time used: 39 seconds