WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3498404
total bases: 503770176
Q20 bases: 494197596(98.0998%)
Q30 bases: 479099575(95.1028%)

Read1 after filtering:
total reads: 3494956
total bases: 503271097
Q20 bases: 493839033(98.1258%)
Q30 bases: 478794597(95.1365%)

Filtering result:
reads passed filter: 3494956
reads failed due to low quality: 623
reads failed due to too many N: 2825
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 86.6014%

JSON report: CypressIslandWA_FG108.json
HTML report: CypressIslandWA_FG108.html

fastp -i CypressIslandWA_FG108.F.fq.gz -o CypressIslandWA_FG108.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG108.json -h CypressIslandWA_FG108.html 
fastp v0.20.0, time used: 39 seconds