WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 2897787
total bases: 417281328
Q20 bases: 409537875(98.1443%)
Q30 bases: 397280235(95.2068%)

Read1 after filtering:
total reads: 2894852
total bases: 416856248
Q20 bases: 409236489(98.1721%)
Q30 bases: 397024409(95.2425%)

Filtering result:
reads passed filter: 2894852
reads failed due to low quality: 661
reads failed due to too many N: 2274
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 87.0574%

JSON report: CypressIslandWA_FG103-B.json
HTML report: CypressIslandWA_FG103-B.html

fastp -i CypressIslandWA_FG103-B.F.fq.gz -o CypressIslandWA_FG103-B.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG103-B.json -h CypressIslandWA_FG103-B.html 
fastp v0.20.0, time used: 33 seconds