WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
No adapter detected for read1

Read1 before filtering:
total reads: 3921069
total bases: 564633936
Q20 bases: 553895634(98.0982%)
Q30 bases: 536995162(95.105%)

Read1 after filtering:
total reads: 3917143
total bases: 564066240
Q20 bases: 553481434(98.1235%)
Q30 bases: 536639109(95.1376%)

Filtering result:
reads passed filter: 3917143
reads failed due to low quality: 651
reads failed due to too many N: 3275
reads failed due to too short: 0
reads with adapter trimmed: 0
bases trimmed due to adapters: 0

Duplication rate (may be overestimated since this is SE data): 84.433%

JSON report: CypressIslandWA_FG101-B.json
HTML report: CypressIslandWA_FG101-B.html

fastp -i CypressIslandWA_FG101-B.F.fq.gz -o CypressIslandWA_FG101-B.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CypressIslandWA_FG101-B.json -h CypressIslandWA_FG101-B.html 
fastp v0.20.0, time used: 42 seconds