WARNING: cut_by_quality5 is deprecated, please use cut_front instead. Detecting adapter sequence for read1... No adapter detected for read1 Read1 before filtering: total reads: 3761216 total bases: 541615104 Q20 bases: 530942754(98.0295%) Q30 bases: 514719987(95.0343%) Read1 after filtering: total reads: 3756358 total bases: 540912716 Q20 bases: 530478258(98.071%) Q30 bases: 514331083(95.0858%) Filtering result: reads passed filter: 3756358 reads failed due to low quality: 1844 reads failed due to too many N: 3014 reads failed due to too short: 0 reads with adapter trimmed: 0 bases trimmed due to adapters: 0 Duplication rate (may be overestimated since this is SE data): 86.3028% JSON report: CatalinaCA_Q356.json HTML report: CatalinaCA_Q356.html fastp -i CatalinaCA_Q356.F.fq.gz -o CatalinaCA_Q356.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j CatalinaCA_Q356.json -h CatalinaCA_Q356.html fastp v0.20.0, time used: 39 seconds