WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
AGAAGACTCAAAGCGAACCAAACAGGCAAAAAATTTAGGGTCGGCATCAAAAGCAATATC

Read1 before filtering:
total reads: 4092711
total bases: 589350384
Q20 bases: 572457723(97.1337%)
Q30 bases: 549086359(93.1681%)

Read1 after filtering:
total reads: 4061359
total bases: 584770916
Q20 bases: 569083180(97.3173%)
Q30 bases: 546065957(93.3812%)

Filtering result:
reads passed filter: 4061359
reads failed due to low quality: 10690
reads failed due to too many N: 5621
reads failed due to too short: 15041
reads with adapter trimmed: 25197
bases trimmed due to adapters: 2226787

Duplication rate (may be overestimated since this is SE data): 63.8768%

JSON report: SewardAK_Q324.json
HTML report: SewardAK_Q324.html

fastp -i SewardAK_Q324.F.fq.gz -o SewardAK_Q324.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q324.json -h SewardAK_Q324.html 
fastp v0.20.0, time used: 44 seconds