WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
TTTATCCTTTGGATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTA

Read1 before filtering:
total reads: 9831744
total bases: 1415771136
Q20 bases: 1374610142(97.0927%)
Q30 bases: 1319021197(93.1663%)

Read1 after filtering:
total reads: 9771049
total bases: 1405879395
Q20 bases: 1367753624(97.2881%)
Q30 bases: 1313059165(93.3977%)

Filtering result:
reads passed filter: 9771049
reads failed due to low quality: 27650
reads failed due to too many N: 13328
reads failed due to too short: 19717
reads with adapter trimmed: 83117
bases trimmed due to adapters: 3162575

Duplication rate (may be overestimated since this is SE data): 71.0509%

JSON report: SewardAK_Q323.json
HTML report: SewardAK_Q323.html

fastp -i SewardAK_Q323.F.fq.gz -o SewardAK_Q323.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q323.json -h SewardAK_Q323.html 
fastp v0.20.0, time used: 90 seconds