WARNING: cut_by_quality5 is deprecated, please use cut_front instead.
Detecting adapter sequence for read1...
TTTTTGTTAACGTATTTAGCCACATAGAAACCAACAGCCATATAACTGGTAGCTTTAAGC

Read1 before filtering:
total reads: 2204930
total bases: 317509920
Q20 bases: 308135124(97.0474%)
Q30 bases: 295665574(93.1201%)

Read1 after filtering:
total reads: 2180762
total bases: 313907020
Q20 bases: 305268240(97.248%)
Q30 bases: 293057283(93.358%)

Filtering result:
reads passed filter: 2180762
reads failed due to low quality: 6377
reads failed due to too many N: 3099
reads failed due to too short: 14692
reads with adapter trimmed: 33349
bases trimmed due to adapters: 2237478

Duplication rate (may be overestimated since this is SE data): 78.2897%

JSON report: SewardAK_Q318.json
HTML report: SewardAK_Q318.html

fastp -i SewardAK_Q318.F.fq.gz -o SewardAK_Q318.R1.fq.gz --cut_by_quality5 20 --cut_by_quality3 20 --cut_window_size 5 --cut_mean_quality 15 -q 15 -u 50 -j SewardAK_Q318.json -h SewardAK_Q318.html 
fastp v0.20.0, time used: 27 seconds